Lauryl Sulfate Broth is sterilized before use for:

The correct procedure for sterilizing Lauryl Sulfate Broth involves maintaining the broth at a temperature of 121 degrees Celsius for a duration of 15 minutes. This temperature and time combination effectively ensures that the broth is free of viable microorganisms, enabling it to serve as a reliable medium for bacterial detection and isolation in subsequent tests.

At 121 degrees Celsius, steam under pressure is used, which is particularly effective at destroying bacterial spores and other resistant forms of microorganisms. The 15-minute duration is generally sufficient to achieve sterilization, assuming a proper autoclave environment is maintained. This practice aligns with standard sterilization protocols for media used in microbiological labs, where contamination must be minimized to ensure accurate results.

Options suggesting shorter or longer times or different temperatures do not meet the established sterilization requirements needed for effective use of Lauryl Sulfate Broth, which is crucial for applications such as water quality testing, where false positives or negatives may significantly impact the results.